Coding

Part:BBa_K2121004:Design

Designed by: Jinny van Doorn   Group: iGEM16_Yale   (2016-10-20)


Lambda_Beta_F7X3D1 (red_rhizo-MAGE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 536
    Illegal NgoMIV site found at 914
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Elimination of any illegal restriction sites, and codon optimization for expression.


Source

Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11). Gene: bet, Ordered Locus Names:SM11_chr1058

References